ARG56564
anti-CRB3 antibody [14F9]
anti-CRB3 antibody [14F9] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rat Monoclonal antibody [14F9] recognizes CRB3 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 14F9 |
| Isotype | IgG2c |
| Target Name | CRB3 |
| Antigen Species | Human |
| Immunogen | Full length of Human CRB3 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Protein crumbs homolog 3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified. |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CRB3 |
| Gene Full Name | crumbs family member 3 |
| Background | This gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions. [UniProt] |
| Calculated MW | 13 kDa |
Images (4) Click the Picture to Zoom In
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ARG56564 anti-CRB3 antibody [14F9] ICC/IF image
Immunofluorescence: Overexpressed human CRB3 in lysates of HeLa cells stained with ARG56564 anti-CRB3 antibody [14F9].
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ARG56564 anti-CRB3 antibody [14F9] WB image
Western blot: analysis of full length CRB3 (GST-CRB3) but not the extracellular domain of CRB3 (GST-CRB3extra). ARG56564 anti-CRB3 antibody [14F9] was used at 1:1000 dilution.
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ARG56564 anti-CRB3 antibody [14F9] ICC/IF image
Immunofluorescence: Endogenous human CRB3 expression in the mouse intestine stained with ARG56564 anti-CRB3 antibody [14F9]. DAPI was used to stain the nuclei in HeLa cells, whereas E-cadherin staining was used to highlight the lateral membrane in mouse intestine.
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ARG56564 anti-CRB3 antibody [14F9] WB image
Western blot: analysis of overexpressed (HEK293T cells) and endogenous CRB3 (mouse tissues). The blots were stained with ARG56564 anti-CRB3 antibody [14F9].
