ARG63563
anti-CPEB1 antibody
anti-CPEB1 antibody for Western blot and Human
Gene Regulation antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CPEB1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise alle reported isoforms. Variants (NP_001073002.1; NP_001073003.1) encode the same isoform. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CPEB1 |
| Antigen Species | Human |
| Immunogen | C-PLMRNQKNRDSS |
| Conjugation | Un-conjugated |
| Alternate Names | hCPEB-1; CPEB-1; CPE-BP1; CPE-binding protein 1; CPEB; Cytoplasmic polyadenylation element-binding protein 1; h-CPEB |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9BZB8 Human Cytoplasmic polyadenylation element-binding protein 1 |
|---|---|
| Background | This gene encodes a member of the cytoplasmic polyadenylation element (CPE) binding protein family. This highly conserved protein binds to a specific RNA sequence called the CPE found in the 3' UTR of some mRNAs. Similar proteins in Xenopus and mouse function to induce cytoplasmic polyadenylation of dormant mRNAs with short polyA tails, resulting in their translation. Members of this protein family regulate translation of cyclin B1 during embryonic cell divisions. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody; Neuroscience antibody |
| Calculated MW | 63 kDa |
| PTM | Phosphorylated on serine/threonine residues by AURKA within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA (By similarity). |
Images (1) Click the Picture to Zoom In
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ARG63563 anti-CPEB1 antibody WB image
Western Blot: human brain lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63563 anti-CPEB1 antibody at 0.5 µg/ml dilution.
