ARG42101
anti-CNTN1 / Contactin 1 antibody
anti-CNTN1 / Contactin 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Goat Polyclonal antibody recognizes CNTN1 / Contactin 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Dog, Pig |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CNTN1 / Contactin 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human CNTN1 / Contactin 1. (C-QVTSQEYSARLEN) (NP_001834.2; NP_778203.1) |
| Conjugation | Un-conjugated |
| Alternate Names | Contactin-1; F3; Glycoprotein gp135; GP135; Neural cell surface protein F3; MYPCN |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 140 - 150 kDa (glycosylated) |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CNTN1 |
| Gene Full Name | contactin 1 |
| Background | The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| Function | Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth (By similarity). [UniProt] |
| Cellular Localization | Isoform 1: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. [UniProt] |
| Calculated MW | 113 kDa |
Images (4) Click the Picture to Zoom In
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ARG42101 anti-CNTN1 / Contactin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse cerebral cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42101 anti-CNTN1 / Contactin 1 antibody at 5 µg/ml dilution followed by DAB-staining.
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ARG42101 anti-CNTN1 / Contactin 1 antibody WB image
Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG42101 anti-CNTN1 / Contactin 1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG42101 anti-CNTN1 / Contactin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42101 anti-CNTN1 / Contactin 1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG42101 anti-CNTN1 / Contactin 1 antibody WB image
Western blot: 35 µg of Mouse brain and Rat brain lysates (in RIPA buffer) stained with ARG42101 anti-CNTN1 / Contactin 1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
