ARG63479
anti-CHFR antibody
anti-CHFR antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CHFR |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Pig |
| Tested Application | IHC-P |
| Specificity | This antibody is expected to recognise all reported isoforms. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CHFR |
| Antigen Species | Human |
| Immunogen | KFNHICEQTRFKN |
| Conjugation | Un-conjugated |
| Alternate Names | EC 6.3.2.-; RNF196; E3 ubiquitin-protein ligase CHFR; RNF116; Checkpoint with forkhead and RING finger domains protein; RING finger protein 196 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 73 kDa |
| PTM | Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1. Autoubiquitinated; may regulate its cellular level. Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity. |
Images (1) Click the Picture to Zoom In
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ARG63479 anti-CHFR antibody IHC-P image
Immunohistochemistry: paraffin embedded human brain (cerebellum). (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63479 anti-CHFR antibody at 2.5 µg/ml dilution followed by AP-staining.
