ARG65210

anti-CHCHD3 antibody

anti-CHCHD3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes CHCHD3
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Sheep
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CHCHD3
Antigen Species Human
Immunogen KFKRYESHPVCAD
Conjugation Un-conjugated
Alternate Names Coiled-coil-helix-coiled-coil-helix domain-containing protein 3; Mic19; MINOS3; PPP1R22; MICOS complex subunit MIC19

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 54927 Human CHCHD3

Swiss-port # Q9NX63 Human MICOS complex subunit MIC19

Gene Symbol CHCHD3
Gene Full Name coiled-coil-helix-coiled-coil-helix domain containing 3
Function Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. [UniProt]
Research Area Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 26 kDa

Images (3) Click the Picture to Zoom In

  • ARG65210 anti-CHCHD3 antibody WB image

    Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG65210 anti-CHCHD3 antibody at 1 µg/ml dilution.

  • ARG65210 anti-CHCHD3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65210 anti-CHCHD3 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG65210 anti-CHCHD3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65210 anti-CHCHD3 antibody at 5 µg/ml dilution followed by AP-staining.