ARG45228

anti-CEP250 / CNAP1 Polyclonal antibody

anti-CEP250 / CNAP1 Polyclonal antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CEP250 / CNAP1
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CEP250 / CNAP1
Antigen Species Human
Immunogen Recombinant protein containing to human CEP250 / CNAP1.
Conjugation Un-conjugated
Alternate Names CEP250; Centrosomal Protein 250; C-NAP1; CEP2; Centrosomal Nek2-Associated Protein 1; Centrosome-Associated Protein CEP250; 250 KDa Centrosomal Protein; Centrosomal Protein 2; CNAP1; Centrosomal Protein 250kDa; EC 3.1.1.61; EC 2.6.1.9; EC 4.3.2.1; CRDHL2; Cep250; C-Nap1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 250 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11190 Human CEP250

GeneID: 16328 Mouse CEP250

Swiss-port # Q60952 Mouse Centrosome-associated protein CEP250

Swiss-port # Q9BV73 Human Centrosome-associated protein CEP250

Gene Symbol CEP250
Gene Full Name Centrosomal Protein 250
Background This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
Function May be involved in ciliogenesis. [UniProt]
Cellular Localization Cell projection; Cilium; Cytoplasm; Cytoskeleton. [UniProt]
Calculated MW 281 kDa
PTM Phosphoprotein. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45228 anti-CEP250 / CNAP1 Polyclonal antibody IHC-P image

    Immunohistochemistry: Human placenta stained with ARG45228 anti-CEP250 / CNAP1 Polyclonal antibody at 2 μg/ml dilution.

  • ARG45228 anti-CEP250 / CNAP1 Polyclonal antibody WB image

    Western blot: Hela and 293T stained with ARG45228 anti-CEP250 / CNAP1 Polyclonal antibody at 0.5 μg/ml dilution.

  • ARG45228 anti-CEP250 / CNAP1 Polyclonal antibody FACS image

    Flow Cytometry: SiHa stained with ARG45228 anti-CEP250 / CNAP1 Polyclonal antibody at 1 µg/10^6 cells dilution.

  • ARG45228 anti-CEP250 / CNAP1 Polyclonal antibody IHC-P image

    Immunohistochemistry: Rat brain stained with ARG45228 anti-CEP250 / CNAP1 Polyclonal antibody at 2 μg/ml dilution.

  • ARG45228 anti-CEP250 / CNAP1 Polyclonal antibody IHC-P image

    Immunohistochemistry: Mouse brain stained with ARG45228 anti-CEP250 / CNAP1 Polyclonal antibody at 2 μg/ml dilution.