ARG41587
anti-CDC23 antibody
anti-CDC23 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CDC23 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CDC23 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 50-150 of Human CDC23 (NP_004652.2). |
| Conjugation | Un-conjugated |
| Alternate Names | CUT23; Cell division cycle protein 23 homolog; ANAPC8; APC8; Anaphase-promoting complex subunit 8; Cyclosome subunit 8 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Jurkat | ||||||
| Observed Size | ~ 63 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8BGZ4 Mouse Cell division cycle protein 23 homolog Swiss-port # Q9UJX2 Human Cell division cycle protein 23 homolog |
|---|---|
| Gene Symbol | CDC23 |
| Gene Full Name | cell division cycle 23 |
| Background | The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008] |
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt] |
| Calculated MW | 69 kDa |
| PTM | Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG41587 anti-CDC23 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung tissue stained with ARG41587 anti-CDC23 antibody at 1:100 dilution.
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ARG41587 anti-CDC23 antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG41587 anti-CDC23 antibody at 1:1000 dilution.
