ARG41587

anti-CDC23 antibody

anti-CDC23 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CDC23
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CDC23
Antigen Species Human
Immunogen Synthetic peptide within aa. 50-150 of Human CDC23 (NP_004652.2).
Conjugation Un-conjugated
Alternate Names CUT23; Cell division cycle protein 23 homolog; ANAPC8; APC8; Anaphase-promoting complex subunit 8; Cyclosome subunit 8

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat
Observed Size ~ 63 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 52563 Mouse CDC23

GeneID: 8697 Human CDC23

Swiss-port # Q8BGZ4 Mouse Cell division cycle protein 23 homolog

Swiss-port # Q9UJX2 Human Cell division cycle protein 23 homolog

Gene Symbol CDC23
Gene Full Name cell division cycle 23
Background The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008]
Function Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt]
Calculated MW 69 kDa
PTM Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG41587 anti-CDC23 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse lung tissue stained with ARG41587 anti-CDC23 antibody at 1:100 dilution.

  • ARG41587 anti-CDC23 antibody WB image

    Western blot: 25 µg of Jurkat cell lysate stained with ARG41587 anti-CDC23 antibody at 1:1000 dilution.