ARG41845
anti-CDC16 / APC6 antibody
anti-CDC16 / APC6 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CDC16 / APC6 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CDC16 / APC6 |
| Antigen Species | Human |
| Immunogen | An 18 amino acid synthetic peptide within the first 50 amino acids of Human CDC16 / APC6. |
| Conjugation | Un-conjugated |
| Alternate Names | Cell division cycle protein 16 homolog; Cyclosome subunit 6; Anaphase-promoting complex subunit 6; CDC16Hs; CDC16 homolog; APC6; CUT9; ANAPC6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Human liver | ||||||
| Observed Size | ~ 72 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13042 Human Cell division cycle protein 16 homolog |
|---|---|
| Gene Symbol | CDC16 |
| Gene Full Name | cell division cycle 16 |
| Background | This gene encodes a component protein of the APC complex, which is composed of eight proteins and functions as a protein ubiquitin ligase. The APC complex is a cyclin degradation system that governs exit from mitosis. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain that may be involved in protein-protein interaction. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle. [UniProt] |
| Calculated MW | 72 kDa |
| PTM | Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG41845 anti-CDC16 / APC6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG41845 anti-CDC16 / APC6 antibody at 5 µg/ml dilution.
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ARG41845 anti-CDC16 / APC6 antibody WB image
Western blot: Human liver lysate stained with ARG41845 anti-CDC16 / APC6 antibody at 1 and 2 µg/ml dilution.
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ARG41845 anti-CDC16 / APC6 antibody IHC image
Immunohistochemistry: Human liver tissue stained with ARG41845 anti-CDC16 / APC6 antibody at 20 µg/ml dilution.
