ARG54208

anti-CD90 / Thy 1 antibody [5E10] (PE)

anti-CD90 / Thy 1 antibody [5E10] (PE) for Flow cytometry and Human,Dog,Primates,Pig

Developmental Biology antibody; Immune System antibody; Neuroscience antibody
publication_link Publication1

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [5E10] recognizes CD90 / Thy 1
Tested Reactivity Hu, Dog, NHuPrm, Pig
Tested Application FACS
Specificity The clone 5E10 recognizes CD90/Thy-1, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells and neurons.
Host Mouse
Clonality Monoclonal
Clone 5E10
Isotype IgG1
Target Name CD90 / Thy 1
Immunogen HEL erythroleukemia cells.
Conjugation PE
Alternate Names Thy-1 membrane glycoprotein; Thy-1 antigen; CD antigen CD90; CDw90; CD90

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7070 Human THY1

Swiss-port # P04216 Human Thy-1 membrane glycoprotein

Gene Symbol THY1
Gene Full Name Thy-1 cell surface antigen
Background CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.
Function May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. [UniProt]
Highlight Related products:
CD90 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
Stem cell and the regenerative medicine: Ready for the patients
Research Area Developmental Biology antibody; Immune System antibody; Neuroscience antibody
Calculated MW 18 kDa

Images (1) Click the Picture to Zoom In

  • ARG54208 anti-CD90 / Thy 1 antibody [5E10] (PE) FACS image

    Flow Cytometry: Separation of Jurkat cells (red-filled) from SP2 cells (black-dashed). Cells were stained with ARG54208 anti-CD90 / Thy 1 antibody [5E10] (PE) (10 µl reagent / 10^6 cells in 100 µl of cell suspension).

Specific References

Tissue Harvesting Site Effect on the Canine Adipose Stromal Vascular Fraction Quantity and Quality.

FACS / Dog

Hanan Hendawy et al.
Animals (Basel).,  (2021)

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