ARG23177

anti-CD8b antibody [PPT23] (PE)

anti-CD8b antibody [PPT23] (PE) for Flow cytometry and Pig

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [PPT23] recognizes CD8b
Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine α/β T cells. Characterization of clone PPT23 has shown that in thymic tissue both CD8lo and CD8hi cells are recognized (Yang H & Parkhouse R.M. 1997). Inhibition studies have demonstrated that clone PPT23 recognizes a different epitope of on the CD8 beta chain to clone PPT22.
Tested Reactivity Pig
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone PPT23
Isotype IgG1
Target Name CD8b
Antigen Species Pig
Immunogen Porcine thymus membrane lysate.
Conjugation PE
Alternate Names LY3; CD8B1; CD antigen CD8b; LEU2; T-cell surface glycoprotein CD8 beta chain; P37; LYT3

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose.
Preservative 0.09% Sodium azide
Stabilizer 1% BSA and 5% Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD8B
Gene Full Name CD8b molecule
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]
Function Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. [UniProt]
Highlight Related products:
CD8 antibodies; CD8 ELISA Kits; CD8 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Tumor-Infiltrating Lymphocytes (TILs)
Calculated MW 24 kDa
PTM Phosphorylated as a consequence of T-cell activation. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG23177 anti-CD8b antibody [PPT23] (PE) FACS image

    Flow Cytometry: Porcine peripheral blood lymphocytes stained with ARG23177 anti-CD8b antibody [PPT23] (PE).

  • ARG23177 anti-CD8b antibody [PPT23] (PE) FACS image

    Flow Cytometry: Figure A. FITC-conjugated Mouse anti Porcine CD5 and PE-conjugated Mouse IgG1 isotype control. Figure B. FITC-conjugated Mouse anti Porcine CD5 and ARG23177 anti-CD8b antibody [PPT23] (PE). All experiments performed on red cell lysed Porcine blood gated on mononuclear cells.

  • ARG23177 anti-CD8b antibody [PPT23] (PE) FACS image

    Flow Cytometry: Figure A. ARG23177 anti-CD8b antibody [PPT23] (PE) and FITC-conjugated Mouse IgG2a isotype control. Figure B. ARG23177 anti-CD8b antibody [PPT23] (PE) and FITC-conjugated Mouse anti Porcine CD5. All experiments performed on red cell lysed Porcine blood gated on mononuclear cells.