ARG23177
anti-CD8b antibody [PPT23] (PE)
anti-CD8b antibody [PPT23] (PE) for Flow cytometry and Pig
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [PPT23] recognizes CD8b Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine α/β T cells. Characterization of clone PPT23 has shown that in thymic tissue both CD8lo and CD8hi cells are recognized (Yang H & Parkhouse R.M. 1997). Inhibition studies have demonstrated that clone PPT23 recognizes a different epitope of on the CD8 beta chain to clone PPT22. |
|---|---|
| Tested Reactivity | Pig |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PPT23 |
| Isotype | IgG1 |
| Target Name | CD8b |
| Antigen Species | Pig |
| Immunogen | Porcine thymus membrane lysate. |
| Conjugation | PE |
| Alternate Names | LY3; CD8B1; CD antigen CD8b; LEU2; T-cell surface glycoprotein CD8 beta chain; P37; LYT3 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA and 5% Sucrose |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CD8B |
|---|---|
| Gene Full Name | CD8b molecule |
| Background | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010] |
| Function | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. [UniProt] |
| Highlight | Related products: CD8 antibodies; CD8 ELISA Kits; CD8 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| Calculated MW | 24 kDa |
| PTM | Phosphorylated as a consequence of T-cell activation. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG23177 anti-CD8b antibody [PPT23] (PE) FACS image
Flow Cytometry: Porcine peripheral blood lymphocytes stained with ARG23177 anti-CD8b antibody [PPT23] (PE).
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ARG23177 anti-CD8b antibody [PPT23] (PE) FACS image
Flow Cytometry: Figure A. FITC-conjugated Mouse anti Porcine CD5 and PE-conjugated Mouse IgG1 isotype control. Figure B. FITC-conjugated Mouse anti Porcine CD5 and ARG23177 anti-CD8b antibody [PPT23] (PE). All experiments performed on red cell lysed Porcine blood gated on mononuclear cells.
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ARG23177 anti-CD8b antibody [PPT23] (PE) FACS image
Flow Cytometry: Figure A. ARG23177 anti-CD8b antibody [PPT23] (PE) and FITC-conjugated Mouse IgG2a isotype control. Figure B. ARG23177 anti-CD8b antibody [PPT23] (PE) and FITC-conjugated Mouse anti Porcine CD5. All experiments performed on red cell lysed Porcine blood gated on mononuclear cells.
