ARG22727
anti-CD8b antibody [CA15.4G2]
anti-CD8b antibody [CA15.4G2] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Dog
Overview
| Product Description | Mouse Monoclonal antibody [CA15.4G2] recognizes CD8b This antibody recognizes Canine CD8β cell surface antigen, expressed by cytotoxic\suppressor T lymphocytes that interact with MHC Class I expressing targets. |
|---|---|
| Tested Reactivity | Dog |
| Tested Application | FACS, IHC-Fr, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CA15.4G2 |
| Isotype | IgG1 |
| Target Name | CD8b |
| Antigen Species | Dog |
| Conjugation | Un-conjugated |
| Alternate Names | LY3; CD8B1; CD antigen CD8b; LEU2; T-cell surface glycoprotein CD8 beta chain; P37; LYT3 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | FACS: Use 20 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Unpurified. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CD8B |
|---|---|
| Gene Full Name | CD8b molecule |
| Background | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010] |
| Function | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. [UniProt] |
| Highlight | Related products: CD8 antibodies; CD8 ELISA Kits; CD8 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| Calculated MW | 24 kDa |
| PTM | Phosphorylated as a consequence of T-cell activation. |
Images (2) Click the Picture to Zoom In
-
ARG22727 anti-CD8b antibody [CA15.4G2] FACS image
Flow Cytometry: Figure A. anti-Canine CD4 (A488) and Mouse IgG1 isotype control detected with Goat anti-Mouse IgG1 DyLight 649. Figure B. anti-Canine CD4 (A488) and ARG22727 anti-CD8b antibody [CA15.4G2] detected with Goat anti-Mouse IgG1 (DyLight 649). All experiments performed on red cell lysed Canine blood gated on mononuclear cells.
-
ARG22727 anti-CD8b antibody [CA15.4G2] FACS image
Flow Cytometry: Figure A. ARG22727 anti-CD8b antibody [CA15.4G2] detected with Goat anti-Mouse IgG1 (DyLight 649) and Rat IgG2b isotype control (FITC). Figure B. ARG22727 anti-CD8b antibody [CA15.4G2] detected with Goat anti-Mouse IgG1 (DyLight 649) and anti-Canine CD4 (FITC). All experiments performed on red cell lysed Canine blood gated on mononuclear cells.
