ARG62941

anti-CD8a antibody [53-6.7] (PE)

anti-CD8a antibody [53-6.7] (PE) for Blocking,Flow cytometry,ICC/IF,IHC-Frozen sections and Mouse

Developmental Biology antibody; Immune System antibody; Cytotoxic T antibody; Cytotoxic T Cell Surface Study antibody; Tumor-infiltrating Lymphocyte Study antibody
publication_link Publication1

Overview

Product Description PE-conjugated Rat Monoclonal antibody [53-6.7] recognizes CD8a
Tested Reactivity Ms
Tested Application BL, FACS, ICC/IF, IHC-Fr
Specificity This antibody recognizes mouse CD8a (32-34 kDa; alpha chain of the CD8 antigen).
Host Rat
Clonality Monoclonal
Clone 53-6.7
Isotype IgG2a, kappa
Target Name CD8a
Antigen Species Mouse
Immunogen Spleen cells or thymocyte membranes
Conjugation PE
Alternate Names T-cell surface glycoprotein CD8 alpha chain; Leu2; p32; T-lymphocyte differentiation antigen T8/Leu-2; CD8; MAL; CD antigen CD8a

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 0.2 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Buffer PBS, 0.1% Sodium azide and Sucrose.
Preservative 0.1% Sodium azide
Stabilizer Sucrose
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12525 Mouse CD8A

Swiss-port # P01731 Mouse T-cell surface glycoprotein CD8 alpha chain

Gene Symbol CD8A
Gene Full Name CD8 antigen, alpha chain
Background CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Function CD8 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. [UniProt]
Highlight Related products:
CD8 antibodies; CD8 ELISA Kits; CD8 Duos / Panels; Anti-Rat IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Detecting exosomal HMGB1 for ICD research
Research Area Developmental Biology antibody; Immune System antibody; Cytotoxic T antibody; Cytotoxic T Cell Surface Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Calculated MW 26 kDa
PTM All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.

Images (5) Click the Picture to Zoom In

  • ARG62941 anti-CD8a antibody [53-6.7] (PE) FACS image

    Flow Cytometry: C57BL/6 Mouse splenocytes stained with ARG62941 anti-CD8a antibody [53-6.7] (PE) and anti-CD4 antibody (FITC).

  • ARG62941 anti-CD8a antibody [53-6.7] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21980 anti-CD4 antibody [GK1.5] (Biotin) and ARG62941 anti-CD8a antibody [53-6.7] (PE).

  • ARG62941 anti-CD8a antibody [53-6.7] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21981 anti-CD4 antibody [GK1.5] (Spectral Red) and ARG62941 anti-CD8a antibody [53-6.7] (PE).

  • ARG62941 anti-CD8a antibody [53-6.7] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21983 anti-CD4 antibody [GK1.5] (PE/Cy5.5) and ARG62941 anti-CD8a antibody [53-6.7] (PE).

  • ARG62941 anti-CD8a antibody [53-6.7] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21984 anti-CD4 antibody [GK1.5] (PE-Cy7) and ARG62941 anti-CD8a antibody [53-6.7] (PE).

Specific References

Extracellular acidity in tumor tissue upregulates programmed cell death protein 1 expression on tumor cells via proton-sensing G protein-coupled receptors

ICC/IF / Mouse

Daichi Mori. et al.
Int J Cancer.,  (2021)

publication_link

 

hr_line