ARG62941
anti-CD8a antibody [53-6.7] (PE)
anti-CD8a antibody [53-6.7] (PE) for Blocking,Flow cytometry,ICC/IF,IHC-Frozen sections and Mouse
Developmental Biology antibody; Immune System antibody; Cytotoxic T antibody; Cytotoxic T Cell Surface Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Publication1
Overview
| Product Description | PE-conjugated Rat Monoclonal antibody [53-6.7] recognizes CD8a |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | BL, FACS, ICC/IF, IHC-Fr |
| Specificity | This antibody recognizes mouse CD8a (32-34 kDa; alpha chain of the CD8 antigen). |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 53-6.7 |
| Isotype | IgG2a, kappa |
| Target Name | CD8a |
| Antigen Species | Mouse |
| Immunogen | Spleen cells or thymocyte membranes |
| Conjugation | PE |
| Alternate Names | T-cell surface glycoprotein CD8 alpha chain; Leu2; p32; T-lymphocyte differentiation antigen T8/Leu-2; CD8; MAL; CD antigen CD8a |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Buffer | PBS, 0.1% Sodium azide and Sucrose. |
| Preservative | 0.1% Sodium azide |
| Stabilizer | Sucrose |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01731 Mouse T-cell surface glycoprotein CD8 alpha chain |
|---|---|
| Gene Symbol | CD8A |
| Gene Full Name | CD8 antigen, alpha chain |
| Background | CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| Function | CD8 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. [UniProt] |
| Highlight | Related products: CD8 antibodies; CD8 ELISA Kits; CD8 Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Detecting exosomal HMGB1 for ICD research |
| Research Area | Developmental Biology antibody; Immune System antibody; Cytotoxic T antibody; Cytotoxic T Cell Surface Study antibody; Tumor-infiltrating Lymphocyte Study antibody |
| Calculated MW | 26 kDa |
| PTM | All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. |
Images (5) Click the Picture to Zoom In
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ARG62941 anti-CD8a antibody [53-6.7] (PE) FACS image
Flow Cytometry: C57BL/6 Mouse splenocytes stained with ARG62941 anti-CD8a antibody [53-6.7] (PE) and anti-CD4 antibody (FITC).
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ARG62941 anti-CD8a antibody [53-6.7] (PE) FACS image
Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21980 anti-CD4 antibody [GK1.5] (Biotin) and ARG62941 anti-CD8a antibody [53-6.7] (PE).
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ARG62941 anti-CD8a antibody [53-6.7] (PE) FACS image
Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21981 anti-CD4 antibody [GK1.5] (Spectral Red) and ARG62941 anti-CD8a antibody [53-6.7] (PE).
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ARG62941 anti-CD8a antibody [53-6.7] (PE) FACS image
Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21983 anti-CD4 antibody [GK1.5] (PE/Cy5.5) and ARG62941 anti-CD8a antibody [53-6.7] (PE).
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ARG62941 anti-CD8a antibody [53-6.7] (PE) FACS image
Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21984 anti-CD4 antibody [GK1.5] (PE-Cy7) and ARG62941 anti-CD8a antibody [53-6.7] (PE).
Specific References
Extracellular acidity in tumor tissue upregulates programmed cell death protein 1 expression on tumor cells via proton-sensing G protein-coupled receptors
ICC/IF / Mouse
