ARG52811

anti-CD79a antibody

Datasheet

Ordering Information

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Price (USD)

500 μl

379.00

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Datasheet

Overview

Product Description	Rabbit Polyclonal antibody recognizes CD79a
Tested Reactivity	Hu
Predict Reactivity	Dog
Tested Application	IHC-P
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	CD79a
Antigen Species	Human
Immunogen	Synthetic peptide derived from N-terminus of human CD79a protein.
Conjugation	Un-conjugated
Alternate Names	Surface IgM-associated protein; B-cell antigen receptor complex-associated protein alpha chain; Membrane-bound immunoglobulin-associated protein; Ig-alpha; MB-1 membrane glycoprotein; MB-1; IGA; CD antigen CD79a

Application Instructions

Application Suggestion

Tested Application	Dilution
IHC-P	1:200

Application Note

IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control

B Cell Lymphoma, Tonsil, Raji

Properties

Form	Liquid
Purification	Immunogen affinity purified
Buffer	PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative	< 0.1% Sodium azide
Stabilizer	1% BSA
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 973 Human CD79A Swiss-port # P11912 Human B-cell antigen receptor complex-associated protein alpha chain
Background	CD79a: The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Function	CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells. [UniProt]
Cellular Localization	Membrane
Highlight	Related products: CD79a antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Lymphoma Tumor-Infiltrating Lymphocytes (TILs)
Research Area	Cancer antibody; Developmental Biology antibody; Immune System antibody
Calculated MW	25 kDa
PTM	Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylated by LYN. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation. Arginine methylation in the ITAM domain may interfere with the binding of SYK. It promotes signals leading to B-cell differentiation (By similarity).