ARG23387

anti-CD68 antibody [ED1]

Datasheet

Publication1

Ordering Information

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Price (USD)

100 μg

355.00

50 μg

245.00

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Overview

Product Description	Mouse Monoclonal antibody [ED1] recognizes CD68 Mouse anti rat CD68, clone ED1 recognizes the rat ED1 antigen. The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat.
Tested Reactivity	Ms, Rat, Bov
Species Does Not React With	Hrs
Tested Application	FACS, IHC-Fr, IHC-P, IP, WB
Host	Mouse
Clonality	Monoclonal
Clone	ED1
Isotype	IgG1
Target Name	CD68
Antigen Species	Rat
Immunogen	Rat spleen cells
Conjugation	Un-conjugated
Alternate Names	Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	Neat - 1:10
IHC-Fr	Assay-dependent
IHC-P	Assay-dependent
IP	Assay-dependent
WB	Assay-dependent

Application Note

FACS: Membrane permeabilisation is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
IHC-P: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purification with Protein A.
Buffer	PBS and 0.09% Sodium azide.
Preservative	0.09% Sodium azide
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 12514 Mouse CD68 Swiss-port # P31996 Mouse Macrosialin
Gene Symbol	CD68
Gene Full Name	CD68 molecule
Background	CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Function	CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]
Highlight	Related products: CD68 antibodies; CD68 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH
Research Area	Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
Calculated MW	37 kDa
PTM	N- and O-glycosylated. [UniProt]

Images (8) Click the Picture to Zoom In

ARG23387 anti-CD68 antibody [ED1] WB image

Western blot: 20 µg of Rat spleen lysate stained with ARG23387 anti-CD68 antibody [ED1] at 1:500 dilution.
ARG23387 anti-CD68 antibody [ED1] IHC image

Immunohistochemistry: Rat liver, with induced hepatocellular damage, stained with ARG23387 anti-CD68 antibody [ED1].
ARG23387 anti-CD68 antibody [ED1] IHC-Fr image

Immunohistochemistry: Rat lymph node cryosection stained with ARG23387 anti-CD68 antibody [ED1] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (Low power).
ARG23387 anti-CD68 antibody [ED1] IHC-Fr image

Immunohistochemistry: Rat lymph node cryosection stained with ARG23387 anti-CD68 antibody [ED1] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (Medium power).
ARG23387 anti-CD68 antibody [ED1] IHC-Fr image

Immunohistochemistry: Rat lymph node cryosection stained with ARG23387 anti-CD68 antibody [ED1] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (High power).
ARG23387 anti-CD68 antibody [ED1] IHC-Fr image

Immunohistochemistry: Rat lymph node cryosection stained with ARG23387 anti-CD68 antibody [ED1], red in A and Mouse anti Rat CD4, green in B. C is the merged image with nuclei counter-stained blue using DAPI. (Low power).
ARG23387 anti-CD68 antibody [ED1] IHC-Fr image

Immunohistochemistry: Rat lymph node cryosection stained with ARG23387 anti-CD68 antibody [ED1], red in A and Mouse anti Rat CD4, green in B. C is the merged image with nuclei counter-stained blue using DAPI. (Medium power).
ARG23387 anti-CD68 antibody [ED1] IHC-Fr image

Immunohistochemistry: Rat lymph node cryosection stained with ARG23387 anti-CD68 antibody [ED1], red in A and Mouse anti Rat CD4, green in B. C is the merged image with nuclei counter-stained blue using DAPI. (High power).