ARG43649

anti-CD68 antibody

anti-CD68 antibody for ICC/IF,Western blot and Human,Mouse,Rat

Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes CD68
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD68
Antigen Species Human
Immunogen Recombinant protein fragment within a.a. 150-350 of Human CD68.
Conjugation Un-conjugated
Protein Full Name Macrosialin
Alternate Names Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 80-130 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration Batch dependent
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12514 Mouse CD68

GeneID: 968 Human CD68

Swiss-port # P31996 Mouse Macrosialin

Swiss-port # P34810 Human Macrosialin

Gene Symbol CD68
Gene Full Name CD68 molecule
Background CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human Polycytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Function CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]
Cellular Localization Cell membrane; Endosome; Lysosome; Membrane
Highlight Related Antibody Duos and Panels:
ARG30325 Inflammatory Cell Antibody Panel
Related products:
CD68 antibodies; CD68 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
Calculated MW 37 kDa
PTM N- and O-glycosylated. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG43649 anti-CD68 antibody ICC/IF image

    Immunofluorescence: THP-1 cells stained with ARG43649 anti-CD68 antibody at 1:250 dilution.

  • ARG43649 anti-CD68 antibody WB image

    Western blot: Mouse liver and Rat liver stained with ARG43649 anti-CD68 antibody at 1:10000 dilution, overnight at 4°C.

  • ARG43649 anti-CD68 antibody WB image

    Western blot: RAW264.7 cell lysates stained with ARG43649 anti-CD68 antibody at 1:10000 dilution, overnight at 4°C.

Specific References

Isoxanthohumol reduces neointimal hyperplasia through the apelin/AKT pathway

WB / Rat

Ting-Yu Chang et al.
Biochim Biophys Acta Mol Basis Dis.,  (2024)

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