ARG43671
anti-CD50 / ICAM3 antibody [ICAM3/1019]
anti-CD50 / ICAM3 antibody [ICAM3/1019] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [ICAM3/1019] recognizes CD50 / ICAM3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ICAM3/1019 |
| Isotype | IgG2b, kappa |
| Target Name | CD50 / ICAM3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to CD50 / ICAM3 antibody. |
| Conjugation | Un-conjugated |
| Protein Full Name | Intercellular adhesion molecule 3 |
| Alternate Names | CD50; CD antigen CD50; Intercellular adhesion molecule 3; ICAM-3; ICAM-R; CDw50; CDW50 |
Application Instructions
| Application Suggestion |
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| Application Note | Antigen retrieval for IHC-P: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 100-160 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ICAM3 |
| Gene Full Name | intercellular adhesion molecule 3 |
| Background | The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008] |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. [UniProt] |
| Cellular Localization | Cell surface |
| Calculated MW | 60 kDa |
| PTM | Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. |
Images (2) Click the Picture to Zoom In
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ARG43671 anti-CD50 / ICAM3 antibody [ICAM3/1019] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human Tonsil tissue stained with ARG43671 anti-CD50 / ICAM3 antibody [ICAM3/1019] at 1:100 dilution. Antigen Retrieval: Heat mediated was performed using 10 mM citrate buffer pH 6.0
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ARG43671 anti-CD50 / ICAM3 antibody [ICAM3/1019] WB image
Western blot: HL-60 cell lysate ARG43671 anti-CD50 / ICAM3 antibody [ICAM3/1019] at 1:100 dilution
