ARG66341
anti-CD3e antibody [SQab1860]
anti-CD3e antibody [SQab1860] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1860] recognizes CD3e |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1860 |
| Isotype | IgG |
| Target Name | CD3e |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of CD3e. |
| Conjugation | Un-conjugated |
| Alternate Names | T-cell surface antigen T3/Leu-4 epsilon chain; T3E; TCRE; T-cell surface glycoprotein CD3 epsilon chain; IMD18; CD antigen CD3e |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Molt-4, Human tonsil tissue | ||||||||
| Observed Size | 23 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07766 Human T-cell surface glycoprotein CD3 epsilon chain |
|---|---|
| Gene Symbol | CD3E |
| Gene Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
| Background | CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. |
| Function | CD3: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). [UniProt] |
| Highlight | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody |
| Calculated MW | 23 kDa |
Images (3) Click the Picture to Zoom In
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ARG66341 anti-CD3e antibody [SQab1860] WB image (Customer's Feedback)
Western blot: Molt-4 cell lysate stained with ARG66341 anti-CD3e antibody [SQab1860] at 1:1000 dilution.
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ARG66341 anti-CD3e antibody [SQab1860] FACS image
Flow Cytometry: Raji cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66341 anti-CD3e antibody [SQab1860] (red) at 1:10 in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66341 anti-CD3e antibody [SQab1860] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue stained with ARG66341 anti-CD3e antibody [SQab1860] at 1:200 dilution. Antigen Retrieval: Heat mediated tissue section in Tris/EDTA buffer (pH 9.0).
Customer's Feedback
Excellent
Review for anti-CD3e antibody [SQab1860]
Application：WB
Sample：Molt-4
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：Overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
