ARG41443
anti-CD3D antibody
anti-CD3D antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD3D |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD3D |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human CD3D. |
| Conjugation | Un-conjugated |
| Alternate Names | CD3D; CD3 Delta Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Delta Chain; CD3-DELTA; CD3DELTA; T3D; CD3d Antigen, Delta Polypeptide (TiT3 Complex); CD3d Molecule, Delta (CD3-TCR Complex); T-Cell Receptor T3 Delta Chain; CD3 Antigen, Delta Subunit; OKT3, Delta Chain; CD3d Molecule; CD3d Antigen; CD3 Delta; IMD19 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| Positive Control | Jurkat | ||||||||||||
| Observed Size | ~ 19 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P04234 Human T-cell surface glycoprotein CD3 delta chain |
|---|---|
| Gene Symbol | CD3D |
| Gene Full Name | CD3 Delta Subunit Of T-Cell Receptor Complex |
| Background | The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. |
| Cellular Localization | Cell membrane, Membrane |
| Highlight | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| Calculated MW | 19 kDa |
| PTM | Disulfide bond, Glycoprotein, Phosphoprotein |
Images (3) Click the Picture to Zoom In
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ARG41443 anti-CD3D antibody ICC/IF image
Immunofluorescence: Jurkat cells stained with ARG41443 anti-CD3D antibody.
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ARG41443 anti-CD3D antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue stained with ARG41443 anti-CD3D antibody.
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ARG41443 anti-CD3D antibody WB image
Western blot: Jurkat cell lysate stained with ARG41443 anti-CD3D antibody.
