ARG55402

anti-CD34 antibody [581] (FITC)

anti-CD34 antibody [581] (FITC) for Flow cytometry and Human,Primates

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [581] recognizes CD34
Tested Reactivity Hu, NHuPrm
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone 581
Isotype IgG1
Target Name CD34
Antigen Species Human
Immunogen Human CD34 protein
Conjugation FITC
Alternate Names Hematopoietic progenitor cell antigen CD34; CD antigen CD34

Application Instructions

Application Suggestion
Tested Application Dilution
FACS4 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 947 Human CD34

Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34

Gene Symbol CD34
Gene Full Name CD34 molecule
Background CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]
Highlight Related products:
CD34 antibodies; CD34 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Stem cell and the regenerative medicine: Ready for the patients
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
Calculated MW 41 kDa
PTM Highly glycosylated.
Phosphorylated on serine residues by PKC.

Images (3) Click the Picture to Zoom In

  • ARG55402 anti-CD34 antibody [581] (FITC) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG55402 anti-CD34 antibody [581] (FITC) (4 µl reagent / 100 µl of peripheral whole blood).

  • ARG55402 anti-CD34 antibody [581] (FITC) FACS image

    Flow Cytometry: Separation of human CD34 positive stem cells (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG55402 anti-CD34 antibody [581] (FITC) (4 µl reagent / 100 µl of peripheral whole blood).

  • ARG55402 anti-CD34 antibody [581] (FITC) FACS image

    Flow Cytometry: Human peripheral whole blood showing CD34 positive stem cells (red). Cells were stained with ARG55402 anti-CD34 antibody [581] (FITC) (4 µl reagent / 100 µl of peripheral whole blood).