ARG54315

anti-CD33 antibody [WM53] (PerCP)

anti-CD33 antibody [WM53] (PerCP) for Flow cytometry and Human,Primates

Developmental Biology antibody; Immune System antibody; Human MDSC Marker antibody; Myeloid-derived suppressor cell antibody

Overview

Product Description PerCP-conjugated Mouse Monoclonal antibody [WM53] recognizes CD33
Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The clone WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
HLDA IV; WS Code M-505
Host Mouse
Clonality Monoclonal
Clone WM53
Isotype IgG1
Target Name CD33
Antigen Species Human
Immunogen Human AML cells
Conjugation PerCP
Alternate Names p67; Sialic acid-binding Ig-like lectin 3; SIGLEC-3; CD antigen CD33; gp67; Siglec-3; Myeloid cell surface antigen CD33; SIGLEC3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 945 Human CD33

Swiss-port # P20138 Human Myeloid cell surface antigen CD33

Gene Symbol CD33
Gene Full Name CD33 molecule
Background CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
Function CD33: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323). [UniProt]
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Research Area Developmental Biology antibody; Immune System antibody; Human MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Calculated MW 40 kDa
PTM Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.

Images (1) Click the Picture to Zoom In

  • ARG54315 anti-CD33 antibody [WM53] (PerCP) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG54315 anti-CD33 antibody [WM53] (PerCP).

Clone References

Highly multiparametric analysis by mass cytometry.

FACS / Human

Ornatsky O et al.
J Immunol Methods.,  (2010)

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A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.

Hernández-Caselles T et al.
J Leukoc Biol.,  (2006)

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Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function.

Leone AM et al.
Eur Heart J.,  (2005)

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Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine.

Schenk M et al.
J Immunol.,  (2005)

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Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells.

Garnache-Ottou F et al.
Blood.,  (2005)

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