ARG65443
anti-CD3 zeta phospho (Tyr72) antibody [EM-26]
anti-CD3 zeta phospho (Tyr72) antibody [EM-26] for Flow cytometry,ICC/IF,Western blot and Human,Mouse
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [EM-26] recognizes CD3 zeta phospho (Tyr72) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, WB |
| Specificity | The clone EM-26 recognizes phosphorylated tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | EM-26 |
| Isotype | IgG1 |
| Target Name | CD3 zeta |
| Antigen Species | Mouse |
| Immunogen | A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | IMD25; CD3Z; CD3-ZETA; T-cell surface glycoprotein CD3 zeta chain; CD3Q; T-cell receptor T3 zeta chain; CD3H; TCRZ; T3Z; CD antigen CD247 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Non-reducing condition recommended. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | FACS: Jurkat + pervanadate; T-cells from lymph nodes of OT-1 mouse + pervanadate. WB: Jurkat + pervanadate; Splenocyte of Balb/c or F1 mouse + pervanadate. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P20963 Human T-cell surface glycoprotein CD3 zeta chain Swiss-port # P24161 Mouse T-cell surface glycoprotein CD3 zeta chain |
|---|---|
| Gene Symbol | Cd247 |
| Gene Full Name | CD247 antigen |
| Background | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. |
| Function | Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. [UniProt] |
| Highlight | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 19 kDa |
| PTM | Phosphorylated on Tyr residues after T-cell receptor triggering. |
Images (2) Click the Picture to Zoom In
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ARG65443 anti-CD3 zeta phospho (Tyr72) antibody [EM-26] WB image
Western blot: Cell lysates with various phosphorylated particular Human CD3 zeta mutants stained with ARG65443 anti-CD3 zeta phospho (Tyr72) antibody [EM-26].
Y1F mutatants lack phosphotyrosine 72, and Y6F mutatants lack phosphotyrosine 153. -
ARG65443 anti-CD3 zeta phospho (Tyr72) antibody [EM-26] ICC/IF image
Immunofluorescence: Immunological synapse formed between the lymph node naïve T cells from TCR transgenic mice and DCEK cells treated with MCC peptide for 20 min stained with ARG65443 anti-CD3 zeta phospho (Tyr72) antibody [EM-26] (light blue)
Actin cytoskeleton (red).
