ARG65722
anti-CD3 zeta phospho (Tyr142) antibody [EM-54]
anti-CD3 zeta phospho (Tyr142) antibody [EM-54] for Flow cytometry,Western blot and Human,Mouse
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [EM-54] recognizes CD3 zeta phospho (Tyr142) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, WB |
| Specificity | This antibody recognizes phosphorylated tyrosine 142 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | EM-54 |
| Isotype | IgG1 |
| Target Name | CD3 zeta |
| Antigen Species | Human |
| Immunogen | Phosphospecific peptide around Tyr142 of Human CD3 zeta. |
| Conjugation | Un-conjugated |
| Alternate Names | IMD25; CD3Z; CD3-ZETA; T-cell surface glycoprotein CD3 zeta chain; CD3Q; T-cell receptor T3 zeta chain; CD3H; TCRZ; T3Z; CD antigen CD247 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P20963 Human T-cell surface glycoprotein CD3 zeta chain Swiss-port # P24161 Mouse T-cell surface glycoprotein CD3 zeta chain |
|---|---|
| Gene Symbol | CD247 |
| Gene Full Name | CD247 molecule |
| Background | The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. [UniProt] |
| Highlight | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 19 kDa |
| PTM | Phosphorylated on Tyr residues after T-cell receptor triggering. |
Images (1) Click the Picture to Zoom In
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ARG65722 anti-CD3 zeta phospho (Tyr142) antibody [EM-54] WB image
Westren blot: Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were stained with ARG65722 anti-CD3 zeta phospho (Tyr142) antibody [EM-54].
Lanes:
1) Wt mCD3zeta pervanadate non-stimulated
2) Wt mCD3zeta pervanadate stimulated
3) mut. mCD3zeta Y/F2-6
4) mut. mCD3zeta Y/F1 and 3-6
5) mut. mCD3zeta Y/F1-2 and 4-6
6) mut. mCD3zeta Y/F1-3 and 5-6
7) mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native)
8) mut. mCD3zeta Y/F1-5.
