ARG65551

anti-CD3 epsilon antibody [UCHT1] (Biotin)

anti-CD3 epsilon antibody [UCHT1] (Biotin) for Blocking,Cellular activation ,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [UCHT1] recognizes CD3 epsilon
Tested Reactivity Hu
Tested Application BL, Cell-Act , FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone UCHT1
Isotype IgG1, kappa
Target Name CD3 epsilon
Antigen Species Human
Immunogen Human peripheral blood lymphocytes from a patient with Sézary's syndrome and infant thymocytes
Conjugation Biotin
Alternate Names CD3E; CD3 Epsilon Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Epsilon Chain; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); CD3-Epsilon; CD3epsilon

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
Cell-Act Assay-dependent
FACS10 µl/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 916 Human CD3E

Swiss-port # P07766 Human T-cell surface glycoprotein CD3 epsilon chain

Gene Symbol CD3E
Gene Full Name CD3 Epsilon Subunit Of T-Cell Receptor Complex
Background The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways.
Cellular Localization Cell membrane, Membrane
Highlight Related products:
CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Exploring Antiviral Immune Response
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Calculated MW 19 kDa

Images (1) Click the Picture to Zoom In

  • ARG65551 anti-CD3 epsilon antibody [UCHT1] (Biotin) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with ARG65551 anti-CD3 epsilon antibody [UCHT1] (Biotin) and ARG21258 anti-CD19 antibody [SJ25-C1] (FITC) followed by Streptavidin (PE).

Clone References

Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.

Siegers GM et al.
J Exp Med.,  (2007)

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Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.

Senechal B et al.
PLoS Med.,  (2007)

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Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency.

Rieux-Laucat F et al.
N Engl J Med.,  (2006)

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Identification and characterization of human pulmonary dendritic cells.

Demedts IK et al.
Am J Respir Cell Mol Biol.,  (2005)

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Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.

Arnett KL et al.
Proc Natl Acad Sci U S A.,  (2004)

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