ARG62801
anti-CD3 epsilon antibody [OKT3] (low endotoxin)
anti-CD3 epsilon antibody [OKT3] (low endotoxin) for Flow cytometry,Functional study,IHC-Frozen sections and Human
Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Overview
| Product Description | Functional grade and low endotoxin Mouse Monoclonal antibody [OKT3] recognizes CD3 epsilon |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, IHC-Fr |
| Specificity | The clone OKT3 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia. HLDA VI; WS Code T6T-R3 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | OKT3 |
| Isotype | IgG2a |
| Target Name | CD3 epsilon |
| Immunogen | Commercially sensitive information |
| Conjugation | Un-conjugated |
| Alternate Names | CD3E; CD3 Epsilon Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Epsilon Chain; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); CD3-Epsilon; CD3epsilon |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: Counteracting both generation and function of effector T cells. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07766 Human T-cell surface glycoprotein CD3 epsilon chain |
|---|---|
| Gene Symbol | CD3E |
| Gene Full Name | CD3 Epsilon Subunit Of T-Cell Receptor Complex |
| Background | The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. |
| Cellular Localization | Cell membrane, Membrane |
| Highlight | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody |
| Calculated MW | 19 kDa |
Images (2) Click the Picture to Zoom In
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ARG62801 anti-CD3 epsilon antibody [OKT3] (low endotoxin) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG62801 anti-CD3 epsilon antibody [OKT3] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62801 anti-CD3 epsilon antibody [OKT3] (low endotoxin) FACS image
Flow Cytometry: Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG62801 anti-CD3 epsilon antibody [OKT3] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
Clone References
STIM1 is required for attenuation of PMCA-mediated Ca2+ clearance during T-cell activation.
Role for circulating osteogenic precursor cells in aortic valvular disease.
FACS / Human
TCR stimulation with modified anti-CD3 mAb expands CD8+ T cell population and induces CD8+CD25+ Tregs.
