ARG62800

anti-CD3 epsilon antibody [MEM-57] (Biotin)

anti-CD3 epsilon antibody [MEM-57] (Biotin) for Flow cytometry and Human

Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [MEM-57] recognizes CD3 epsilon
Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
HLDA IV.; WS Code T 96
Host Mouse
Clonality Monoclonal
Clone MEM-57
Isotype IgG2a
Target Name CD3 epsilon
Antigen Species Human
Immunogen Human thymocytes and T lymphocytes.
Conjugation Biotin
Alternate Names CD3E; CD3 Epsilon Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Epsilon Chain; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); CD3-Epsilon; CD3epsilon

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 916 Human CD3E

Swiss-port # P07766 Human T-cell surface glycoprotein CD3 epsilon chain

Gene Symbol CD3E
Gene Full Name CD3 Epsilon Subunit Of T-Cell Receptor Complex
Background The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways.
Cellular Localization Cell membrane, Membrane
Highlight Related products:
CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Exploring Antiviral Immune Response
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Calculated MW 19 kDa

Images (2) Click the Picture to Zoom In

  • ARG62800 anti-CD3 epsilon antibody [MEM-57] (Biotin) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG62800 anti-CD3 epsilon antibody [MEM-57] (Biotin) at 4 µg/ml dilution, followed by Streptavidin (APC).

  • ARG62800 anti-CD3 epsilon antibody [MEM-57] (Biotin) FACS image

    Flow Cytometry: Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG62800 anti-CD3 epsilon antibody [MEM-57] (Biotin) at 4 µg/ml dilution, followed by Streptavidin (APC).

Clone References

Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells.

FACS / 

Giese S et al.
PLoS Pathog.,  (2014)

publication_link

 

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Lck mediates signal transmission from CD59 to the TCR/CD3 pathway in Jurkat T cells.

Lipp AM et al.
PLoS One.,  (2014)

publication_link

 

hr_line

Pfit is a structurally novel Crohn's disease-associated superantigen.

Liu L et al.
PLoS Pathog.,  (2013)

publication_link

 

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A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

FACS / Human

Phadwal K et al.
Autophagy.,  (2012)

publication_link

 

hr_line

Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3.

Soucek J et al.
Neoplasma.,  (1991)

publication_link

 

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