ARG21154

anti-CD3 epsilon antibody [CT-3] (PE-Cyanine 5)

anti-CD3 epsilon antibody [CT-3] (PE-Cyanine 5) for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Chicken

Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

Overview

Product Description PE-Cyanine 5-conjugated Mouse Monoclonal antibody [CT-3] recognizes CD3 epsilon
Tested Reactivity Chk
Tested Application FACS, IHC-Fr, IHC-P
Specificity Chicken CD3 epsilon. The clone CT-3 recognizes a complex of at least three polypeptides of Mr 20, 19, and 17 kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90 kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50 and 40 kDa.
Host Mouse
Clonality Monoclonal
Clone CT-3
Isotype IgG1, kappa
Target Name CD3 epsilon
Antigen Species Chicken
Immunogen Chicken thymocytes and Ig- blood mononuclear cells
Conjugation PE-Cyanine 5
Alternate Names CD3E; CD3 Epsilon Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Epsilon Chain; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); CD3-Epsilon; CD3epsilon; T3E; T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3; CD3e Molecule; CD3e Antigen; CD3-EPSILON; CD3EPSILON

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 0.2 µg/10^6 cells
IHC-FrAssay-dependent
IHC-PAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS, 0.1% Sodium azide and Sucrose.
Preservative 0.1% Sodium azide
Stabilizer Sucrose
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD3E
Gene Full Name CD3 Epsilon Subunit Of T-Cell Receptor Complex
Background The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathway.
Cellular Localization Cell membrane, Membrane
Highlight Related products:
CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels;
Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Calculated MW 23 kDa
PTM Disulfide bond, Phosphoprotein

Images (1) Click the Picture to Zoom In

  • ARG21154 anti-CD3 epsilon antibody [CT-3] (PE-Cyanine 5) FACS image

    Flow Cytometry: Chicken peripheral blood lymphocytes stained with ARG21154 anti-CD3 epsilon antibody [CT-3] (PE-Cyanine 5) and ARG21162 anti-CD8a antibody [CT-8] (PE).