ARG23047
anti-CD3 antibody [KT3] (APC)
anti-CD3 antibody [KT3] (APC) for Flow cytometry,IHC-Frozen sections and Mouse
Overview
| Product Description | APC-conjugated Rat Monoclonal antibody [KT3] recognizes CD3 CD3 antibody, clone KT3 recognizes the mouse CD3 antigen expressed by mature T cells. Clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, IHC-Fr |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | KT3 |
| Isotype | IgG2a |
| Target Name | CD3 |
| Antigen Species | Mouse |
| Immunogen | CBAT6 thymocytes. |
| Conjugation | APC |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA and 5% Sucrose |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CD3D, CD3E, CD3G, CD247 |
|---|---|
| Gene Full Name | CD3 Delta Subunit Of T-Cell Receptor Complex, CD3 Epsilon Subunit Of T-Cell Receptor Complex, CD3 Gamma Subunit Of T-Cell Receptor Complex, CD247 Molecule |
| Background | CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with the T-cell receptor (TCR) and the CD3-zeta (ζ-chain) to generate an activation signal in T lymphocytes. The TCR, CD3-zeta, and the other CD3 molecules together constitute the TCR complex. |
| Highlight | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Rat IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response |
| Calculated MW | 20 kDa |
Images (2) Click the Picture to Zoom In
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ARG23047 anti-CD3 antibody [KT3] (APC) FACS image
Flow Cytometry: Figure A. Alexa Fluor 488 conjugated rat anti mouse CD8a and APC conjugated rat IgG2a isotyoe control. Figure B. Alexa Fluor 488 conjugated rat anti mouse CD8a and ARG23047 anti-CD3 antibody [KT3] (APC). All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells.
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ARG23047 anti-CD3 antibody [KT3] (APC) FACS image
Flow Cytometry: Figure A. RPE conjugated Rat anti Mouse CD4 and APC conjugated Rat IgG2a isotype control. Figure B. RPE conjugated Rat anti Mouse CD4 and ARG23047 anti-CD3 antibody [KT3] (APC). All experiments performed on murine splenocytes.
