ARG23047

anti-CD3 antibody [KT3] (APC)

anti-CD3 antibody [KT3] (APC) for Flow cytometry,IHC-Frozen sections and Mouse

Overview

Product Description APC-conjugated Rat Monoclonal antibody [KT3] recognizes CD3
CD3 antibody, clone KT3 recognizes the mouse CD3 antigen expressed by mature T cells. Clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
Tested Reactivity Ms
Tested Application FACS, IHC-Fr
Host Rat
Clonality Monoclonal
Clone KT3
Isotype IgG2a
Target Name CD3
Antigen Species Mouse
Immunogen CBAT6 thymocytes.
Conjugation APC

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50 - 1:100
IHC-FrAssay-dependent
Application Note IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose
Preservative 0.09% Sodium azide
Stabilizer 1% BSA and 5% Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD3D, CD3E, CD3G, CD247
Gene Full Name CD3 Delta Subunit Of T-Cell Receptor Complex, CD3 Epsilon Subunit Of T-Cell Receptor Complex, CD3 Gamma Subunit Of T-Cell Receptor Complex, CD247 Molecule
Background CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with the T-cell receptor (TCR) and the CD3-zeta (ζ-chain) to generate an activation signal in T lymphocytes. The TCR, CD3-zeta, and the other CD3 molecules together constitute the TCR complex.
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Calculated MW 20 kDa

Images (2) Click the Picture to Zoom In

  • ARG23047 anti-CD3 antibody [KT3] (APC) FACS image

    Flow Cytometry: Figure A. Alexa Fluor 488 conjugated rat anti mouse CD8a and APC conjugated rat IgG2a isotyoe control. Figure B. Alexa Fluor 488 conjugated rat anti mouse CD8a and ARG23047 anti-CD3 antibody [KT3] (APC). All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells.

  • ARG23047 anti-CD3 antibody [KT3] (APC) FACS image

    Flow Cytometry: Figure A. RPE conjugated Rat anti Mouse CD4 and APC conjugated Rat IgG2a isotype control. Figure B. RPE conjugated Rat anti Mouse CD4 and ARG23047 anti-CD3 antibody [KT3] (APC). All experiments performed on murine splenocytes.