ARG42338

anti-CD274 / PD-L1 antibody [29E.2A3] (PE)

anti-CD274 / PD-L1 antibody [29E.2A3] (PE) for Flow cytometry and Human,Primates

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [29E.2A3] recognizes CD274 / PD-L1
Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The mouse monoclonal antibody 29E.2A3 recognizes an extracellular epitope of CD274 / PD-L1 (also known as B7-H1), a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including T cell lymphomas, melanomas, and glioblastomas.
Host Mouse
Clonality Monoclonal
Clone 29E.2A3
Isotype IgG2b, kappa
Target Name CD274 / PD-L1
Antigen Species Human
Immunogen Full length Human CD274.
Conjugation PE
Alternate Names Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29126 Human CD274

Swiss-port # Q9NZQ7 Human Programmed cell death 1 ligand 1

Gene Symbol CD274
Gene Full Name CD274 molecule
Background This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Function Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Recycling endosome membrane; Single-pass type I membrane protein. Note=Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation. Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Endomembrane system; Single-pass type I membrane protein. [UniProt]
Highlight Related products:
PD-L1 antibodies; PD-L1 ELISA Kits; Anti-Mouse IgG secondary antibodies;
Related news:
Examining CTL/NK-mediated cytotoxicity by ELISA
Calculated MW 33 kDa

Images (2) Click the Picture to Zoom In

  • ARG42338 anti-CD274 / PD-L1 antibody [29E.2A3] (PE) FACS image

    Flow Cytometry: Human PHA stimulated peripheral blood mononuclear cell suspension stained with ARG42338 anti-CD274 / PD-L1 antibody [29E.2A3] (PE) at 10 µl / 10^6 cells in 100 µl of cell suspension.

  • ARG42338 anti-CD274 / PD-L1 antibody [29E.2A3] (PE) FACS image

    Flow Cytometry: Separation of Human CD274 positive cells (red-filled) from cellular debris (black-dashed). Human PHA stimulated peripheral blood mononuclear cell suspension stained with ARG42338 anti-CD274 / PD-L1 antibody [29E.2A3] (PE) at 10 µl / 10^6 cells in 100 µl of cell suspension.