ARG22562
anti-CD273 / PD-L2 antibody [TY25] (FITC)
anti-CD273 / PD-L2 antibody [TY25] (FITC) for Flow cytometry and Mouse
Overview
| Product Description | FITC-conjugated Rat Monoclonal antibody [TY25] recognizes
CD273 / PD-L2. This antibody recognizes mouse CD273, also known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). CD273 is a 247 amino acid, ~42 kDa member of the B7 family. The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4.CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production.Rat anti Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumoto et al. 2004). Removal of sodium azide is recommended prior to use in functional assays, the use of EQU003 is recommended for this purpose. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | TY25 |
| Isotype | IgG2a |
| Target Name | CD273 / PD-L2 |
| Antigen Species | Mouse |
| Immunogen | B7-DC transfected RAW264.7 cells. |
| Conjugation | FITC |
| Alternate Names | B7DC; bA574F11.2; PDL2; CD antigen CD273; PD-L2; Programmed cell death 1 ligand 2; CD273; Btdc; PDCD1L2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; PD-1 ligand 2; PDCD1 ligand 2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide and 1% BSA. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Pdcd1lg2 |
| Gene Full Name | programmed cell death 1 ligand 2 |
| Function | Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity). [UniProt] |
| Highlight | Related products: PD-L2 antibodies; PD-L2 ELISA Kits; Anti-Rat IgG secondary antibodies; Related news: The best solution for PD-1/PD-L1 research |
| Calculated MW | 31 kDa |
