ARG23023

anti-CD25 antibody [OX-39]

anti-CD25 antibody [OX-39] for ELISA,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Rat

Immune System antibody; Pre-B Cell Marker antibody

Overview

Product Description Mouse Monoclonal antibody [OX-39] recognizes CD25
Mouse anti Rat CD25 antibody, clone OX-39 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Paterson et al. 1987).
Tested Reactivity Rat
Tested Application ELISA, FACS, IHC-Fr, IHC-P, IP
Host Mouse
Clonality Monoclonal
Clone OX-39
Isotype IgG1
Target Name CD25
Antigen Species Rat
Immunogen Stimulated Rat T cells
Conjugation Un-conjugated
Alternate Names IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1:10 - 1:50
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
Application Note IHC-P: PLP fixation is recommended.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25704 Rat IL2RA

Swiss-port # P26897 Rat Interleukin-2 receptor subunit alpha

Gene Symbol Il2ra
Gene Full Name interleukin 2 receptor, alpha
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Function Receptor for interleukin-2. [UniProt]
Research Area Immune System antibody; Pre-B Cell Marker antibody
Calculated MW 31 kDa

Images (6) Click the Picture to Zoom In

  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (Low power).

  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (Medium power).

  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (High power).

  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39], red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. (Low power).

  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39], red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. (Medium power).

  • ARG23023 anti-CD25 antibody [OX-39] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23023 anti-CD25 antibody [OX-39], red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. (High power).