ARG40205
anti-CD23 antibody
anti-CD23 antibody for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD23 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD23 |
| Antigen Species | Mouse |
| Immunogen | Recombinant protein corresponding to E50-P331 of Mouse CD23. |
| Conjugation | Un-conjugated |
| Alternate Names | CD23A; BLAST-2; Lymphocyte IgE receptor; CLEC4J; Immunoglobulin E-binding factor; CD23; IGEBF; Fc-epsilon-RII; CD antigen CD23; FCE2; Low affinity immunoglobulin epsilon Fc receptor; C-type lectin domain family 4 member J |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P06734 Human Low affinity immunoglobulin epsilon Fc receptor |
|---|---|
| Gene Symbol | FCER2 |
| Gene Full Name | Fc fragment of IgE, low affinity II, receptor for (CD23) |
| Background | The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] |
| Function | Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Cell membrane; Lipid-anchor. Secreted. Note=Also exists as a soluble excreted form, sCD23. [UniProt] |
| Calculated MW | 36 kDa |
| PTM | N- and O-glycosylated. The secreted form sCD23 is produced by ADAM10-mediated ectodomain shedding. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG40205 anti-CD23 antibody FACS image
Flow Cytometry: H-PBMC cells were blocked with 10% normal goat serum and then stained with ARG40205 anti-CD23 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG40205 anti-CD23 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Mouse spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40205 anti-CD23 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG40205 anti-CD23 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40205 anti-CD23 antibody at 1 µg/ml, overnight at 4°C.
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ARG40205 anti-CD23 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Mouse liver lysate stained with ARG40205 anti-CD23 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG40205 anti-CD23 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40205 anti-CD23 antibody at 1 µg/ml, overnight at 4°C.
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ARG40205 anti-CD23 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40205 anti-CD23 antibody at 1 µg/ml, overnight at 4°C.
