ARG58750

anti-CD223 / LAG3 antibody [1G4]

anti-CD223 / LAG3 antibody [1G4] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Mouse Monoclonal [1G4] antibody recognizes CD223 / LAG3
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 1G4
Isotype IgG1, kappa
Target Name CD223 / LAG3
Antigen Species Human
Immunogen Extracellular domain of Human CD223 / LAG3.
Conjugation Un-conjugated
Alternate Names CD antigen CD223; CD223; Protein FDC; Lymphocyte activation gene 3 protein; LAG-3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1 - 10 µg/ml
IHC-P5 µg/ml
WB0.25 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human lymphoma tissue and spleen tissue.
Observed Size ~ 60, 70, 80 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3902 Human LAG3

Swiss-port # P18627 Human Lymphocyte activation gene 3 protein

Gene Symbol LAG3
Gene Full Name lymphocyte-activation gene 3
Background Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
Function Involved in lymphocyte activation. Binds to HLA class-II antigens. [UniProt]
Calculated MW 57 kDa

Images (5) Click the Picture to Zoom In

  • ARG58750 anti-CD223 / LAG3 antibody [1G4] IF image

    Immunofluorescence: LAG3 over expressing HEK293 cells stained with ARG58750 anti-CD223 / LAG3 antibody [1G4] at 2 µg/ml dilution.

  • ARG58750 anti-CD223 / LAG3 antibody [1G4] IHC image

    Immunohistochemistry: LAG3 over expressing Human spleen tissue stained with ARG58750 anti-CD223 / LAG3 antibody [1G4] at 10 µg/ml dilution.

  • ARG58750 anti-CD223 / LAG3 antibody [1G4] WB image

    Western blot: LAG3 over expressing HEK293 cells stained with ARG58750 anti-CD223 / LAG3 antibody [1G4] at 0.25, 0.5 and 1 µg/ml dilution.

  • ARG58750 anti-CD223 / LAG3 antibody [1G4] ICC image

    Immunocytochemistry: LAG3 over expressing HEK293 cells stained with ARG58750 anti-CD223 / LAG3 antibody [1G4]. Lower left (control): Mouse IgG antibody at 1 µg/ml dilution.

  • ARG58750 anti-CD223 / LAG3 antibody [1G4] IHC-P image

    Immunohistochemistry: Human lymphoma tissue stained with ARG58750 anti-CD223 / LAG3 antibody [1G4]. Lower left (control): Mouse IgG at 5 µg/ml dilution.