ARG65503
anti-CD20 antibody [MEM-97] (azide free)
anti-CD20 antibody [MEM-97] (azide free) for Flow cytometry,Immunoprecipitation and Human,Bovine,Pig
Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody
Overview
| Product Description | Azide free Mouse Monoclonal antibody [MEM-97] recognizes CD20 |
|---|---|
| Tested Reactivity | Hu, Bov, Pig |
| Tested Application | FACS, IP |
| Specificity | The clone MEM-97 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes. HLDA V; WS Code B CD20.9 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-97 |
| Isotype | IgG1 |
| Target Name | CD20 |
| Antigen Species | Human |
| Immunogen | Raji human Burkitt's lymphoma cell line |
| Conjugation | Un-conjugated |
| Alternate Names | Bp35; LEU-16; B-lymphocyte surface antigen B1; B-lymphocyte antigen CD20; CD20; S7; CD antigen CD20; Leukocyte surface antigen Leu-16; B1; CVID5; Membrane-spanning 4-domains subfamily A member 1; MS4A2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MS4A1 |
| Gene Full Name | membrane-spanning 4-domains, subfamily A, member 1 |
| Background | CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008] |
| Function | CD20 is a B-lymphocyte-specific membrane protein. It plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602). [UniProt] |
| Highlight | Related products: CD20 antibodies; CD20 ELISA Kits; CD20 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody |
| Calculated MW | 33 kDa |
| PTM | Phosphorylated. Might be functionally regulated by protein kinase(s). |
Images (2) Click the Picture to Zoom In
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ARG65503 anti-CD20 antibody [MEM-97] (azide free) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG65503 anti-CD20 antibody [MEM-97] (azide free) at 1.6 μg/ml dilution.
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ARG65503 anti-CD20 antibody [MEM-97] (azide free) IP image
Immunoprecipitation: Immunoprecipitation of human CD20 from the whole cell lysate of Raji cells.
1) original lysate of Raji cells, and 2) same cells immunoprecipitate by ARG65503 anti-CD20 antibody [MEM-97] (azide free).
Western blot was stained with anti-CD20 rabbit polyclonal antibody. .
Clone References
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Stimulation of HIV-1 replication in immature dendritic cells in contact with primary CD4 T or B lymphocytes.
FACS / Human
Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY.
