ARG65385
anti-CD20 antibody [2H7] (FITC)
anti-CD20 antibody [2H7] (FITC) for Flow cytometry and Human,Primates
Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody
Overview
| Product Description | FITC-conjugated Mouse Monoclonal antibody [2H7] recognizes CD20 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 3337 kDa nonglycosylated membrane receptor with four transmembrane domains, expressed on preB lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2H7 |
| Isotype | IgG2b |
| Target Name | CD20 |
| Antigen Species | Human |
| Immunogen | Human tonsillar B cells |
| Conjugation | FITC |
| Alternate Names | Bp35; LEU-16; B-lymphocyte surface antigen B1; B-lymphocyte antigen CD20; CD20; S7; CD antigen CD20; Leukocyte surface antigen Leu-16; B1; CVID5; Membrane-spanning 4-domains subfamily A member 1; MS4A2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MS4A1 |
| Gene Full Name | membrane-spanning 4-domains, subfamily A, member 1 |
| Background | CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008] |
| Function | CD20 is a B-lymphocyte-specific membrane protein. It plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602). [UniProt] |
| Highlight | Related products: CD20 antibodies; CD20 ELISA Kits; CD20 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody |
| Calculated MW | 33 kDa |
| PTM | Phosphorylated. Might be functionally regulated by protein kinase(s). |
Images (2) Click the Picture to Zoom In
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ARG65385 anti-CD20 antibody [2H7] (FITC) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG65385 anti-CD20 antibody [2H7] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).
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ARG65385 anti-CD20 antibody [2H7] (FITC) FACS image
Flow Cytometry: Separation of human CD20 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG65385 anti-CD20 antibody [2H7] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).
Clone References
CD20-directed small modular immunopharmaceutical, TRU-015, depletes normal and malignant B cells.
Novel follicular dendritic cell molecule, 8D6, collaborates with CD44 in supporting lymphomagenesis by a Burkitt lymphoma cell line, L3055.
Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.
Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment.
Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20.
