ARG65447

anti-CD163 antibody [GHI/61]

anti-CD163 antibody [GHI/61] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Human

M1/M2/TAM Marker antibody; Macrophage Marker antibody; M2 Macrophage Marker antibody

Overview

Product Description Mouse Monoclonal antibody [GHI/61] recognizes CD163
Tested Reactivity Hu
Tested Application CyTOF®-candidate, FACS, IHC-Fr, IP, WB
Specificity The clone GHI/61 recognizes CD163, an approximately 130 kDa high affinity scavenger receptor expressed mainly on monocytes and macrophages, which binds hemoglobin-haptoglobin complex.
HLDA VI; WS Code M38
Host Mouse
Clonality Monoclonal
Clone GHI/61
Isotype IgG1
Target Name CD163
Immunogen Hairy cell leukemia cells
Conjugation Un-conjugated
Alternate Names sCD163; M130; Scavenger receptor cysteine-rich type 1 protein M130; MM130; CD antigen CD163; Hemoglobin scavenger receptor

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9332 Human CD163

Swiss-port # Q86VB7 Human Scavenger receptor cysteine-rich type 1 protein M130

Gene Symbol CD163
Gene Full Name CD163 molecule
Background CD163 protein is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
Function CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. [UniProt]
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Research Area M1/M2/TAM Marker antibody; Macrophage Marker antibody; M2 Macrophage Marker antibody
Calculated MW 125 kDa
PTM A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Phosphorylated.

Images (2) Click the Picture to Zoom In

  • ARG65447 anti-CD163 antibody [GHI/61] FACS image

    Flow Cytometry: Human peripheral blood stained with ARG65447 anti-CD163 antibody [GHI/61] at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

  • ARG65447 anti-CD163 antibody [GHI/61] FACS image

    Flow Cytometry: Separation of human monocytes (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65447 anti-CD163 antibody [GHI/61] at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

Clone References

Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.

IHC-P / Rabbit

Howard JD et al.
PLoS One.,  (2014)

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A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily.

Law SK et al.
Eur J Immunol.,  (1993)

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A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4.

FACS / 

Pulford K et al.
Immunology.,  (1992)

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