ARG63909
anti-CD14 antibody
anti-CD14 antibody for Western blot and Human
Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CD14 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD14 |
| Antigen Species | Human |
| Immunogen | C-KRVDADADPRQYAD |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08571 Human Monocyte differentiation antigen CD14 |
|---|---|
| Background | The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010] |
| Research Area | Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody |
| Calculated MW | 40 kDa |
| PTM | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
Images (2) Click the Picture to Zoom In
-
ARG63909 anti-CD14 antibody WB image
Western Blot: Human Lymph Node lysate (35 µg protein in RIPA buffer) stained with ARG63909 anti-CD14 antibody at 1 µg/ml dilution.
-
ARG63909 anti-CD14 antibody WB image
Western blot: 35 µg of peripheral blood mononucleocyte lysates (in RIPA buffer) with (B) and without (A) blocking peptide. The blots were stained with ARG63909 anti-CD14 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
