ARG62718

anti-CD11b antibody [M1/70] (Functional Grade)

anti-CD11b antibody [M1/70] (Functional Grade) for CyTOF®-candidate,Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation and Human,Mouse,Primates,Rabbit

MDSC Marker antibody; Myeloid-derived suppressor cell antibody

Overview

Product Description Functional grade and low endotoxin Rat Monoclonal antibody [M1/70] recognizes CD11b
Tested Reactivity Hu, Ms, NHuPrm, Rb
Tested Application CyTOF®-candidate, FACS, FuncSt, IHC-Fr, IP
Specificity The clone M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
Host Rat
Clonality Monoclonal
Clone M1/70
Isotype IgG2b
Target Name CD11b
Antigen Species Mouse
Immunogen B10 mouse spleen cells enriched for T cells
Conjugation Un-conjugated
Alternate Names MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note Functional studies: In vitro blocking of CD11b.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Purification Note 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16409 Mouse ITGAM

GeneID: 3684 Human ITGAM

Swiss-port # P11215 Human Integrin alpha-M

Background CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Function CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt]
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Research Area MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Calculated MW 127 kDa

Images (2) Click the Picture to Zoom In

  • ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) FACS image

    Flow Cytometry: Murine splenocyte suspension stained with ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.

  • ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) FACS image

    Flow Cytometry: Separation of murine CD11b positive myeloid cells (red-filled) from murine CD11b negative lymphocytes (black-dashed). Murine splenocyte suspension stained with ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.

Clone References

Experimental autoimmune prostatitis induces microglial activation in the spinal cord.

Wong L et al.
Prostate.,  (2015)

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Tetrandrine suppresses lipopolysaccharide-induced microglial activation by inhibiting NF-κB and ERK signaling pathways in BV2 cells.

WB / Mouse

Dang Y et al.
PLoS One.,  (2014)

publication_link

 

hr_line

VEGFR-1 blockade disrupts peri-implantation decidual angiogenesis and macrophage recruitment.

IHC / Mouse

Douglas NC et al.
Vasc Cell.,  (2014)

publication_link

 

hr_line

Astrocyte-derived BDNF supports myelin protein synthesis after cuprizone-induced demyelination.

IHC-FoFr / Mouse

Fulmer CG et al.
J Neurosci.,  (2014)

publication_link

 

hr_line

Role of cysteine-rich 61 protein (CCN1) in macrophage-mediated oncolytic herpes simplex virus clearance.

Thorne AH et al.
Mol Ther.,  (2014)

publication_link

 

hr_line

Retinoid-X-receptors (α/β) in melanocytes modulate innate immune responses and differentially regulate cell survival following UV irradiation.

ICC/IF / Mouse

Coleman DJ et al.
PLoS Genet.,  (2014)

publication_link

 

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Gr-1+CD11b+ cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation.

IHC-Fr / Mouse

Liu T et al.
Sci Rep.,  (2014)

publication_link

 

hr_line

Intrastriatal transplantation of adenovirus-generated induced pluripotent stem cells for treating neuropathological and functional deficits in a rodent model of Huntington's disease.

Fink KD et al.
Stem Cells Transl Med.,  (2014)

publication_link

 

hr_line

Neurons are host cells for Mycobacterium tuberculosis.

Randall PJ et al.
Infect Immun.,  (2014)

publication_link

 

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MCP-1/CCR2 signaling-mediated astrocytosis is accelerated in a transgenic mouse model of SOD1-mutated familial ALS.

IHC-FoFr / Mouse

Kawaguchi-Niida M et al.
Acta Neuropathol Commun.,  (2013)

publication_link

 

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Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia.

FACS / Mouse

Olme CH et al.
Leuk Res.,  (2013)

publication_link

 

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Neuronally-expressed Sarm1 regulates expression of inflammatory and antiviral cytokines in brains.

Lin CW et al.
Innate Immun.,  (2014)

publication_link

 

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Matricellular protein CCN1 activates a proinflammatory genetic program in murine macrophages.

FuncSt / Mouse

Bai T et al.
J Immunol.,  (2010)

publication_link

 

hr_line

Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction.

IHC-Fr / Rabbit

Tamagawa E et al.
Am J Physiol Lung Cell Mol Physiol.,  (2008)

publication_link

 

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