ARG62718
anti-CD11b antibody [M1/70] (Functional Grade)
anti-CD11b antibody [M1/70] (Functional Grade) for CyTOF®-candidate,Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation and Human,Mouse,Primates,Rabbit
MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Overview
| Product Description | Functional grade and low endotoxin Rat Monoclonal antibody [M1/70] recognizes CD11b |
|---|---|
| Tested Reactivity | Hu, Ms, NHuPrm, Rb |
| Tested Application | CyTOF®-candidate, FACS, FuncSt, IHC-Fr, IP |
| Specificity | The clone M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | M1/70 |
| Isotype | IgG2b |
| Target Name | CD11b |
| Antigen Species | Mouse |
| Immunogen | B10 mouse spleen cells enriched for T cells |
| Conjugation | Un-conjugated |
| Alternate Names | MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: In vitro blocking of CD11b. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Function | CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt] |
| Highlight | Related products: CD11b antibodies; CD11b Duos / Panels; Anti-Rat Rat secondary antibodies; Related news: CyTOF-candidate Antibodies New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| Research Area | MDSC Marker antibody; Myeloid-derived suppressor cell antibody |
| Calculated MW | 127 kDa |
Images (2) Click the Picture to Zoom In
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ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) FACS image
Flow Cytometry: Murine splenocyte suspension stained with ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.
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ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) FACS image
Flow Cytometry: Separation of murine CD11b positive myeloid cells (red-filled) from murine CD11b negative lymphocytes (black-dashed). Murine splenocyte suspension stained with ARG62718 anti-CD11b antibody [M1/70] (Functional Grade) at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.
Clone References
Experimental autoimmune prostatitis induces microglial activation in the spinal cord.
Tetrandrine suppresses lipopolysaccharide-induced microglial activation by inhibiting NF-κB and ERK signaling pathways in BV2 cells.
WB / Mouse
VEGFR-1 blockade disrupts peri-implantation decidual angiogenesis and macrophage recruitment.
IHC / Mouse
Astrocyte-derived BDNF supports myelin protein synthesis after cuprizone-induced demyelination.
IHC-FoFr / Mouse
Role of cysteine-rich 61 protein (CCN1) in macrophage-mediated oncolytic herpes simplex virus clearance.
Retinoid-X-receptors (α/β) in melanocytes modulate innate immune responses and differentially regulate cell survival following UV irradiation.
ICC/IF / Mouse
Gr-1+CD11b+ cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation.
IHC-Fr / Mouse
Intrastriatal transplantation of adenovirus-generated induced pluripotent stem cells for treating neuropathological and functional deficits in a rodent model of Huntington's disease.
Neurons are host cells for Mycobacterium tuberculosis.
MCP-1/CCR2 signaling-mediated astrocytosis is accelerated in a transgenic mouse model of SOD1-mutated familial ALS.
IHC-FoFr / Mouse
Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia.
FACS / Mouse
Neuronally-expressed Sarm1 regulates expression of inflammatory and antiviral cytokines in brains.
Matricellular protein CCN1 activates a proinflammatory genetic program in murine macrophages.
FuncSt / Mouse
Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction.
IHC-Fr / Rabbit
