ARG65464

anti-CD11b antibody [ICRF44] (Biotin)

anti-CD11b antibody [ICRF44] (Biotin) for Blocking,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Baboon,Chimpanzee,Pig,Rhesus Monkey

MDSC Marker antibody; Myeloid-derived suppressor cell antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [ICRF44] recognizes CD11b
Tested Reactivity Hu, Bb, Chimp, Pig, R. Mk
Tested Application BL, FACS, ICC/IF, IHC-Fr, IP, WB
Host Mouse
Clonality Monoclonal
Clone ICRF44
Isotype IgG1, kappa
Target Name CD11b
Antigen Species Human
Immunogen Rheumatoid synovial cells and human monocytes
Conjugation Biotin
Alternate Names MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS10 µl/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3684 Human ITGAM

Swiss-port # P11215 Human Integrin alpha-M

Gene Symbol ITGAM
Gene Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Background CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Function CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt]
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Research Area MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Calculated MW 127 kDa

Images (1) Click the Picture to Zoom In

  • ARG65464 anti-CD11b antibody [ICRF44] (Biotin) FACS image

    Flow Cytometry: Human peripheral blood granulocytes stained with ARG65464 anti-CD11b antibody [ICRF44] (Biotin) followed by Streptavidin (FITC).

Clone References

Proinflammatory cytokines are involved in the initiation of the abnormal matrix process in pseudoexfoliation syndrome/glaucoma.

IHC-Fr / Human

Zenkel M et al.
Am J Pathol.,  (2010)

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Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins.

BL / Human

Harler MB et al.
J Immunol.,  (1999)

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