ARG23479

anti-CD11b antibody [ED8] (FITC)

anti-CD11b antibody [ED8] (FITC) for Flow cytometry and Rat

MDSC Marker antibody; Myeloid-derived suppressor cell antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [ED8] recognizes CD11b
Mouse anti Rat CD11b antibody, clone ED8 recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes. It also recognizes small ramified microglia in the central nervous system. No other cell types are positive for ED8. The recognized antigen is a heterodimer consisting of ~160 and ~95 kDa, belonging to the family of adhesion molecules CD11b/CD18, also designated as Mac-1 antigen or CR3.
Tested Reactivity Rat
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone ED8
Isotype IgG1
Target Name CD11b
Antigen Species Rat
Immunogen Rat spleen cell homogenate with Freund's complete adjuvant.
Conjugation FITC
Alternate Names MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by ion exchange chromatography.
Buffer PBS, 0.09% Sodium azide and 1% BSA.
Preservative 0.09% Sodium azide
Stabilizer 1% BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25021 Rat ITGAM

Gene Symbol ITGAM
Gene Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Background CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Function CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt]
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Research Area MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Calculated MW 127 kDa

Images (2) Click the Picture to Zoom In

  • ARG23479 anti-CD11b antibody [ED8] (FITC) FACS image

    Flow Cytometry: Figure A. ARG23479 anti-CD11b antibody [ED8] (FITC) and RPE conjugated Mouse IgG1 isotype control. Figure B. ARG23479 anti-CD11b antibody [ED8] (FITC) and RPE conjugated Mouse anti Rat CD106. All experiments performed on red cell lysed Rat bone marrow gated on mononuclear cells.

  • ARG23479 anti-CD11b antibody [ED8] (FITC) FACS image

    Flow Cytometry: Figure A. PE conjugated Mouse anti Rat CD106 and FITC conjugated Mouse IgG2a isotype control. Figure B. PE conjugated Mouse anti Rat CD106 and ARG23479 anti-CD11b antibody [ED8] (FITC). All experiments performed on red cell lysed Rat bone marrow gated on mononuclear cells.