ARG62705
anti-CD11a / LFA1 alpha antibody [MEM-83] (low endotoxin)
anti-CD11a / LFA1 alpha antibody [MEM-83] (low endotoxin) for Flow cytometry,Functional study,Immunoprecipitation and Human
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [MEM-83] recognizes CD11a / LFA1 alpha |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, IP |
| Specificity | The antibody MEM-83 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-83 |
| Isotype | IgG1 |
| Target Name | CD11a / LFA1 alpha |
| Antigen Species | Human |
| Immunogen | Human peripheral blood lymphocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein. |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGAL |
| Gene Full Name | integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) |
| Background | ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is a receptor for F11R (PubMed:11812992, PubMed:15528364). Integin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed:29100055). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992). Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (By similarity). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 129 kDa |
| PTM | In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG62705 anti-CD11a / LFA1 alpha antibody [MEM-83] (low endotoxin) FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG62705 anti-CD11a / LFA1 alpha antibody [MEM-83] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62705 anti-CD11a / LFA1 alpha antibody [MEM-83] (low endotoxin) FACS image
Flow Cytometry: Separation of Human CD11a positive lymphocytes (red-filled) from CD11a negative blood debris (black-dashed). Human peripheral whole blood stained with ARG62705 anti-CD11a / LFA1 alpha antibody [MEM-83] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
Clone References
LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro.
BL /
Regulation of LFA-1-dependent inflammatory cell recruitment by Cbl-b and 14-3-3 proteins.
A novel LFA-1 activation epitope maps to the I domain.
Involvement of the I domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3.
