ARG64958
anti-CD11a / LFA1 alpha antibody
anti-CD11a / LFA1 alpha antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CD11a / LFA1 alpha |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD11a / LFA1 alpha |
| Antigen Species | Human |
| Immunogen | C-YQVRIQPSIHDHN |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA1A; LFA-1A; Integrin alpha-L; CD11A; Leukocyte function-associated molecule 1 alpha chain; LFA-1; CD11 antigen-like family member A; CD antigen CD11a |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Microwaved or steamed tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 129 kDa |
| PTM | In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents. |
Images (3) Click the Picture to Zoom In
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ARG64958 anti-CD11a / LFA1 alpha antibody WB image
Western blot: Human Spleen lysate (35 µg protein in RIPA buffer) stained with ARG64958 anti-CD11a / LFA1 alpha antibody at 0.5 µg/ml dilution.
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ARG64958 anti-CD11a / LFA1 alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lymph node tissue. Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64958 anti-CD11a / LFA1 alpha antibody at 2 µg/ml dilution followed by HRP-staining.
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ARG64958 anti-CD11a / LFA1 alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64958 anti-CD11a / LFA1 alpha antibody at 3.75 µg/ml dilution followed by AP-staining.
