ARG63843
anti-CBX3 / HP1 gamma antibody
anti-CBX3 / HP1 gamma antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CBX3 / HP1 gamma |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Rat |
| Tested Application | IHC-P, WB |
| Specificity | No cross-reactivity expected with HP1-alpha and HP1-beta. NP_009207.2 and NP_057671.2 represent same protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CBX3 / HP1 gamma |
| Antigen Species | Human |
| Immunogen | C-EAFLNSQKAGKEKD |
| Conjugation | Un-conjugated |
| Alternate Names | HP1-GAMMA; Chromobox protein homolog 3; HP1Hs-gamma; HP1 gamma; HECH; Heterochromatin protein 1 homolog gamma; Modifier 2 protein |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011] |
| Highlight | Related products: CBX3 antibodies; CBX3 Duos / Panels; Anti-Goat IgG secondary antibodies; Related news: Senescence Marker Antibody Panel is launched |
| Research Area | Gene Regulation antibody |
| Calculated MW | 21 kDa |
| PTM | Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. |
Images (2) Click the Picture to Zoom In
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ARG63843 anti-CBX3 / HP1 gamma antibody WB image
Western blot: A431 lysate (35 µg protein in RIPA buffer) stained with ARG63843 anti-CBX3 / HP1 gamma antibody at 0.3 µg/ml dilution.
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ARG63843 anti-CBX3 / HP1 gamma antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Placenta. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63843 anti-CBX3 / HP1 gamma antibody at 2.5 µg/ml dilution followed by AP-staining.
