ARG56508
anti-CARD15 / NOD2 antibody
anti-CARD15 / NOD2 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CARD15 / NOD2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CARD15 / NOD2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 28-301 of Human recombinant CARD15 / NOD2. |
| Conjugation | Un-conjugated |
| Alternate Names | Inflammatory bowel disease protein 1; PSORAS1; BLAU; CARD15; CD; CLR16.3; NLRC2; Caspase recruitment domain-containing protein 15; IBD1; ACUG; Nucleotide-binding oligomerization domain-containing protein 2; NOD2B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9HC29 Human Nucleotide-binding oligomerization domain-containing protein 2 |
|---|---|
| Gene Symbol | NOD2 |
| Gene Full Name | nucleotide-binding oligomerization domain containing 2 |
| Background | This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2014] |
| Function | Recognizes muramyl dipeptide (MDP) constituents of bacterial peptidoglycans and plays a key role in gastrointestinal immunity: upon stimulation, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3 and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling, leading to activate the transcription of hundreds of genes involved in immune response. [UniProt] |
| Calculated MW | 115 kDa |
| PTM | Polyubiquitinated following MDP stimulation, leading to proteasome-mediated degradation (PubMed:23019338). |
Images (1) Click the Picture to Zoom In
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ARG56508 anti-CARD15 / NOD2 antibody WB image
Western blot: 1) 0.01 µg of Nod2 Recombinant Fragment, 2) 0.02 µg of Nod2 Recombinant Fragment, 3) 0.03 µg of Nod2 Recombinant Fragment, and 4) 0.05 µg of Nod2 Recombinant Fragment stained with ARG56508 anti-CARD15 / NOD2 antibody.
