ARG63451
anti-CAPZB antibody
anti-CAPZB antibody for Western blot and Human,Mouse,Rat,Pig
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CAPZB |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Cow, Dog |
| Tested Application | WB |
| Specificity | This antibody is expected to be specific for the beta2 isoform of capping protein, found in non-muscle cells and should not recognise the beta1 form. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CAPZB |
| Antigen Species | Human |
| Immunogen | NDLVEALKRKQQC |
| Conjugation | Un-conjugated |
| Alternate Names | CapZ beta; F-actin-capping protein subunit beta; CAPZ; CAPB; CAPPB |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes the beta subunit of the barbed-end actin binding protein, which belongs to the F-actin capping protein family. The capping protein is a heterodimeric actin capping protein that blocks actin filament assembly and disassembly at the fast growing (barbed) filament ends and functions in regulating actin filament dynamics as well as in stabilizing actin filament lengths in muscle and nonmuscle cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, Apr 2011] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 31 kDa |
Images (2) Click the Picture to Zoom In
-
ARG63451 anti-CAPZB antibody WB image
Western blot: 35 µg of Jurkat (A), HeLa (B) and NIH/3T3 (C) cell lysates (in RIPA buffer) stained with ARG63451 anti-CAPZB antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
-
ARG63451 anti-CAPZB antibody WB image
Western blot: 35 µg of Mouse (A), Rat (B) and Pig (C) heart lysates (in RIPA buffer) stained with ARG63451 anti-CAPZB antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
