ARG62692
anti-Blood Group Lewis a antibody [7LE]
anti-Blood Group Lewis a antibody [7LE] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody [7LE] recognizes Blood Group Lewis a |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, WB |
| Specificity | The clone 7LE recognizes Lewisa blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewisa may be useful for detection of gastrointestinal, pancreatic and colorectal tumors. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7LE |
| Isotype | IgG1 |
| Target Name | Blood Group Lewis a |
| Immunogen | Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification). |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Heat mediated antigen retrieval (sodium citrate); mAb incubated for 1 hour at RT; Detection DAB * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-P: Colorectal adenocarcinoma |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Research Area | Cell Biology and Cellular Response antibody |
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Images (3) Click the Picture to Zoom In
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ARG62692 anti-Blood Group Lewis a antibody [7LE] IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon adenocarcinoma tissue stained with ARG62692 anti-Blood Group Lewis a antibody [7LE].
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ARG62692 anti-Blood Group Lewis a antibody [7LE] IHC-P image
Immunohistochemistry: Colorectal adenocarcinoma section stained with ARG62692 anti-Blood Group Lewis a antibody [7LE].
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ARG62692 anti-Blood Group Lewis a antibody [7LE] IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreas tissue stained with ARG62692 anti-Blood Group Lewis a antibody [7LE].
Clone References
Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses.
Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells.
Virus-induced transcriptional activation of host FUT genes associated with neo-expression of Ley in cytomegalovirus-infected and sialyl-Lex in varicella-zoster virus-infected diploid human cells.
Alteration of sialyl Lewis epitope expression in pterygium.
Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens.
Lewis antigen alterations in gastric cancer precursors.
