ARG63723
anti-BOULE / BOLL antibody
anti-BOULE / BOLL antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes BOULE / BOLL |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_932074.1; NP_149019.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BOULE / BOLL |
| Antigen Species | Human |
| Immunogen | C-QPEPIKTVWSIHY |
| Conjugation | Un-conjugated |
| Alternate Names | BOULE; Protein boule-like |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 31 kDa |
Images (1) Click the Picture to Zoom In
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ARG63723 anti-BOULE / BOLL antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Testis. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63723 anti-BOULE / BOLL antibody at 4 µg/ml dilution followed by HRP-staining.
