ARG66957
anti-BDNF antibody
anti-BDNF antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes BDNF |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BDNF |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the middle region of Human BDNF. |
| Conjugation | Un-conjugated |
| Alternate Names | Brain-derived neurotrophic factor; Abrineurin; BULN2; ANON2; BDNF |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse cerebellum; SHSY5Y | ||||||||
| Observed Size | ~ 28 kDa (full length); ~15 kDa (mature) |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300, 20% Glycerol and 1% BSA. |
| Preservative | 0.025% ProClin 300 |
| Stabilizer | 20% Glycerol and 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BDNF |
| Gene Full Name | brain-derived neurotrophic factor |
| Background | The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009] |
| Function | During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. [UniProt] |
| Cellular Localization | Extracellular region or secreted. [UniProt] |
| Highlight | Related products: BDNF antibodies; BDNF ELISA Kits; BDNF recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| Calculated MW | 28 kDa |
| PTM | The propeptide is N-glycosylated and glycosulfated. Converted into mature BDNF by plasmin (PLG). [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG66957 anti-BDNF antibody IHC-Fr image
Immunofluorescence: HuVEC cells were fixed with 4% paraformaldehyde at RT for 10 min, permeabilized with 0.1% NP-40 at RT for 10 min and then cells were blocked with 5% BSA at RT for 30 min and stained with ARG66957 anti-BDNF antibody (red) at 1:200 dilution at 4°C for overnight. DAPI (blue) was used for nuclear staining.
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ARG66957 anti-BDNF antibody IHC-Fr image
Immunohistochemistry: Frozen section of Mouse cerebellum tissue stained with ARG66957 anti-BDNF antibody (green) at 1:200 dilution.
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ARG66957 anti-BDNF antibody WB image
Western blot: 80 µg of SHSY5Y cell lysate stained with ARG66957 anti-BDNF antibody at 1:1000 dilution, overnight at 4°C.
