ARG43079
anti-BCKDK antibody
anti-BCKDK antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes BCKDK |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BCKDK |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 21-70 of Human BCKDK. |
| Conjugation | Un-conjugated |
| Alternate Names | BCKD-kinase; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; BDK; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKDHKIN; BCKDKD; EC 2.7.11.4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.2), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BCKDK |
| Gene Full Name | branched chain ketoacid dehydrogenase kinase |
| Background | The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012] |
| Function | Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex. [UniProt] |
| Cellular Localization | Mitochondrion matrix. Mitochondrion. [UniProt] |
| Calculated MW | 46 kDa |
| PTM | Autophosphorylated. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG43079 anti-BCKDK antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil carcinoma tissue stained with ARG43079 anti-BCKDK antibody at 1:50 dilution.
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ARG43079 anti-BCKDK antibody WB image
Western blot: 40 µg of C6, AML-12, HEK293T and L-02 whole cell lysates stained with ARG43079 anti-BCKDK antibody at 1:500 dilution.
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ARG43079 anti-BCKDK antibody IP image
Immunoprecipitation: HEK293T cell lysate using ARG43079 anti-BCKDK antibody (lane 2 and 3) and Nonspecific IgG Control (lane 4 and 5). Lane 1 is 30% input. Western blot was stained with ARG43079 anti-BCKDK antibody.
↑: Supernatant
↓: Deposition
