ARG63449

anti-BCAP31 / BAP31 antibody

anti-BCAP31 / BAP31 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Cell Death antibody

Overview

Product Description Goat Polyclonal antibody recognizes BCAP31 / BAP31
Tested Reactivity Hu
Predict Reactivity Cow, Dog, Pig
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise isoform a (NP_001132929.1) and isoform b (NP_005736.3; NP_001132913.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name BCAP31 / BAP31
Antigen Species Human
Immunogen C-QAAVDGPMDKKEE
Conjugation Un-conjugated
Alternate Names BAP31; Bap31; 6C6-AG; DXS1357E; CDM; BCR-associated protein 31; 6C6-AG tumor-associated antigen; p28; Protein CDM; B-cell receptor-associated protein 31; DDCH

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10134 Human BCAP31

Swiss-port # P51572 Human B-cell receptor-associated protein 31

Background This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody
Calculated MW 28 kDa
PTM Cleaved by CASP8 and other caspases.

Images (4) Click the Picture to Zoom In

  • ARG63449 anti-BCAP31 / BAP31 antibody WB image

    Western Blot: Human Breast lysate (35 µg protein in RIPA buffer) stained with ARG63449 anti-BCAP31 / BAP31 antibody at 0.1 µg/ml dilution.

  • ARG63449 anti-BCAP31 / BAP31 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63449 anti-BCAP31 / BAP31 antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG63449 anti-BCAP31 / BAP31 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63449 anti-BCAP31 / BAP31 antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG63449 anti-BCAP31 / BAP31 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63449 anti-BCAP31 / BAP31 antibody at 3.75 µg/ml dilution followed by AP-staining.