ARG64147
anti-APPL1 antibody
anti-APPL1 antibody for Western blot and Rat
Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes APPL1 |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Cow, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | APPL1 |
| Antigen Species | Human |
| Immunogen | C-NRYSRLSKKRENDK |
| Conjugation | Un-conjugated |
| Alternate Names | Dip13-alpha; Adapter protein containing PH domain, PTB domain and leucine zipper motif 1; APPL; DIP13alpha; DCC-interacting protein 13-alpha |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008] |
|---|---|
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 80 kDa |
| PTM | Phosphorylation at Ser-410 by PKA severely impairs binding to OCRL. |
Images (1) Click the Picture to Zoom In
-
ARG64147 anti-APPL1 antibody WB image
Western Blot: 1) Rat Aortic Smooth Muscle cell lysate 2) after infection with Human APPL1 adenovirus for 48hrs. stained with ARG64147 anti-APPL1 antibody at 0.5 µg/ml dilution.
